OSMAC Strategy Integrated with Molecular Networking for Accessing Griseofulvin Derivatives from Endophytic Fungi of Moquiniastrum polymorphum (Asteraceae).

Victor F Farinella,Leticia V Costa-Lotufo,Helori V Domingos,Marcelo M P Tangerina,Eunizinis S Kawafune,Marcelo J P Ferreira

doi:10.3390/molecules26237316

Victor F Farinella, Leticia V Costa-Lotufo + Show 4 more

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https://doi.org/10.3390/molecules26237316

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Abstract

Three endophytic fungi isolated from Moquiniastrum polymorphum (Less.) G. Sancho (Asteraceae) were cultivated using the one strain many compounds (OSMAC) strategy to evaluate the production of griseofulvin derivatives. Extracts obtained were analyzed by HPLC–MS/MS and the chromatographic and spectrometric data used to elaborate a feature-based molecular network (FBMN) through the GNPS platform. This approach allowed the observation of differences such as medium-specific and strain-specific production of griseofulvin derivatives and variations of cytotoxic activity in most extracts. To evaluate the efficiency of the OSMAC approach allied with FBMN analysis in the prospection of compounds of biotechnological interest, griseofulvin and 7-dechlorogriseofulvin were isolated, and the relative concentrations were estimated in all culture media using HPLC–UV, allowing for the inference of the best strain–medium combinations to maximize its production. Malt extract-peptone broth and Wickerham broth media produced the highest concentrations of both secondary metabolites.

Highlights

The Moquiniastrum genus (Asteraceae) contains 21 species distributed in South America and 19 species endemics to Brazil [1]
Eighty-six strains of endophytic fungi were recovered from Moquiniastrum polymorphum and codified as MPO574 to MPO660
These strains were cultured on potato dextrose agar (PDA) at 25 ◦C for 28 days and, subsequently, extracted with ethyl acetate

Summary

Introduction

The Moquiniastrum genus (Asteraceae) contains 21 species distributed in South America and 19 species endemics to Brazil [1]. Known as cambará, is a medium-sized tree found in eastern Brazil, as well as Argentina, Paraguay, and Uruguay [2]. Ethnobotanical studies have reported the use of M. polymorphum leaves in infusions to treat sore throat [3]. Phytochemical studies showed the presence of sesquiterpene lactones, diterpenes, triterpenes and coumarins [4,5,6,7]. Within the secondary metabolites described, sesquiterpene lactones presented anti-inflammatory and antineoplastic activities in vivo [2,8]. Studies of the endophytic microbiota have been not described for Moquiniastrum genus in the literature

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