Osj10gBTF3-Mediated Import of Chloroplast Protein Is Essential for Pollen Development in Rice.

Xue-Jiao Liu,Peng Zheng,Hao Chen,Jiaqi Sun,Chao Li,Mehmood Jan,Jumin Tu,Huanquan Zheng,Hao Du,Yue Yuan,Yuqing Huang

doi:10.3389/fpls.2021.713544

Xue-Jiao Liu, Peng Zheng + Show 9 more

Open Access

https://doi.org/10.3389/fpls.2021.713544

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Abstract

Chloroplasts are crucial organelles for the generation of fatty acids and starch required for plant development. Nascent polypeptide-associated complex (NAC) proteins have been implicated in development as transcription factors. However, their chaperone roles in chloroplasts and their relationship with pollen development in plants remain to be elucidated. Here, we demonstrated that Osj10gBTF3, a NAC protein, regulates pollen and chloroplast development in rice by coordinating with a Hsp90 family chaperone OsHSP82 to mediate chloroplast import. Knockout of Osj10gBTF3 affects pollen and chloroplast development and significantly reduces the accumulation of fertility-related chloroplast protein OsPPR676. Both Osj10gBTF3 and OsHSP82 interact with OsPPR676. Interestingly, the interaction between OsHSP82 and OsPPR676 is only found in the cytoplasm, while the interaction between Osj10gBTF3 and OsPPR676 also occurs inside the chloroplast. The chloroplast stroma chaperone OsCpn60 can also be co-precipitated with Osj10gBTF3, but not with OsHSP82. Further investigation indicates that Osj10gBTF3 enters the chloroplast stroma possibly through the inner chloroplast membrane channel protein Tic110 and then recruits OsCpn60 for the folding or assembly of OsPPR676. Our results reveal a chaperone role of Osj10gBTF3 in chloroplast import different from Hsp90 and provide a link between chloroplast transport and pollen development in rice.

Highlights

Chloroplast is the center for energy production
We reveal a novel role of Osj10gBTF3 in the import of chloroplast proteins by using OsPPR676 as a chloroplast protein marker, contributing to a better understanding of the import mechanism of chloroplast proteins, and provide a new insight to pollen development in rice
Phenotype showed that the btf3-1, btf3-2, and btf3-3 mutant plants were slightly shorter when compared with wild-type (WT, left; Figures 1B–D)

Summary

Introduction

A significant proportion of nuclear genes have been identified to be involved in the complex process of chloroplast biogenesis, such as protein translocation (Bauer et al, 2000; Motohashi et al, 2001) and assembly (Sundberg et al, 1997). NAC has been identified as a component of ribosome-associated chaperones which promote the folding of newly synthesized proteins (Preissler and Deuerling, 2012) and the loss of NAC and HSP70 homologs result in substantial growth defects, suggesting that NAC may be connected with the chaperone system (Koplin et al, 2010)

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