Abstract
To develop treatments for neurodegenerative disorders, it is critical to understand the biology and function of neurons in both normal and diseased states. Molecular studies of neurons involve the delivery of small biomolecules into cultured neurons via transfection to study genetic variants. However, as cultured primary neurons are sensitive to temperature change, stress, and shifts in pH, these factors make biomolecule delivery difficult, particularly non-viral delivery. Herein we used oscillating nanomagnetic gene transfection to successfully transfect SH-SY5Y cells as well as primary hippocampal and cortical neurons on different days in vitro. This novel technique has been used to effectively deliver genetic material into various cell types, resulting in high transfection efficiency and viability. From these observations and other related studies, we suggest that oscillating nanomagnetic gene transfection is an effective method for gene delivery into hard-to-transfect neuronal cell types.
Highlights
Neurons are key units of the central nervous system (CNS) which process and transmit information through electrical and chemical signalling [1,2]
Neuronal cell lines are used, for example SH-SY5Y, which is a cell line derived from human neuroblastoma, which can be differentiated into neuronal-like cells with properties of adult neurons [16,17]
SH-SY5Y has served as a model for studying neuron biology, especially in neurodegenerative diseases such as Alzheimer’s disease (AD) and Parkinson’s disease (PD) [18,19,20], and transfection experiments have been conducted on SH-SY5Y to understand neuron damage and degeneration [21], growth and regeneration [22], and the role of nuclear location sequences in non-viral transfection [23]
Summary
Neurons are key units of the central nervous system (CNS) which process and transmit information through electrical and chemical signalling [1,2]. Dysfunction of these critical physiological processes can lead to a host of neurological diseases such as Alzheimer’s disease (AD) [3], Parkinson’s disease (PD) [4], epilepsy [5], and multiple sclerosis [6]. To better understand the biology and function of neurons in the brain, current research involves the delivery of small biomolecules such as DNA, RNA or peptides into cultured neurons by means of transfection to enhance or silence the expression of genes. SH-SY5Y has served as a model for studying neuron biology, especially in neurodegenerative diseases such as AD and PD [18,19,20], and transfection experiments have been conducted on SH-SY5Y to understand neuron damage and degeneration [21], growth and regeneration [22], and the role of nuclear location sequences in non-viral transfection [23]
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