Abstract
A survey of entomopathogenic nematodes was conducted in sugar cane crops in a total of 14 soils, and positive results were obtained for strain MC5-2014 in the municipality of Tepalcingo, Morelos, in soil with a sandy loam texture and a pH of 6.4. Species determination was performed via morphological and morphometric techniques by searching for a tubular stoma with a swollen cylindrical pharyngeal body and a metacorpus in the basal part. The range of body length (L) was 750 to 1200 μm in females and 720 to 910 μm in males, while the corresponding maximum widths (W) of the body were 30 to 60 μm and 20 to 30 μm, respectively. Males exhibited bursa with a 1 + 1 + 3 + 3 distribution of papillae, and females exhibited a vulva located at the mid-body. For molecular identification, the ITS region of ribosomal DNA was used. Virulence tests (LC50) were conducted with Galleria mellonella, and a value of 4.732 was obtained for infective juveniles (IJs). Taking taxonomic and molecular characteristics into account, the isolate was determined to be Oscheius myriophila. The isolation of this strain represents the first geographic report of O. myriophila in Mexico, and it should be noted that the cultivation of sugar cane occurs with constant application of insecticides, herbicides, fungicides, and fertilizers as well as harvesting activities such as burning of the crop for harvest. The O. myriophila isolate has the potential to be used in the future as a method of biological control in our country.
Highlights
Hunt, 2007; Abebe et al, 2010)
In 2017, it was reported that O. myriophila was associated with the European mole cricket, Gryllotalpa gryllotalpa (Orthoptera: Gryllotalpidae) (Zeynep et al, 2017)
Soil samples were collected from 14 plots of sugar cane located in the state of Tepalcingo, Morelos in Mexico using the technique proposed by Stock et al (1999), and a modified method described by Orozco et al (2014)
Summary
The genus Oscheius was described as a sister taxon of Rhabditis (Dujardin, 1845) Sudhaus (1976). The genus Oscheius belongs to the Rhabditidae family and is divided into two groups: insectivora and dolichura. The species O. carolinensis, O. amsactae, O. niazii, and O. siddiqii of the insectivora group and one species of the dolichura group, O. onirici, have been recognized as EPNs (Tabassum and Shahina, 2010; Ye et al, 2010; Torrini et al, 2015). In 2017, it was reported that O. myriophila was associated with the European mole cricket, Gryllotalpa gryllotalpa (Orthoptera: Gryllotalpidae) (Zeynep et al, 2017). This work provides the first geographic report of Oscheius myriophila in Mexico and the first global report of its entomopathogenic nature
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