OSC21: Morphological, Mechanical and Biological Properties of Silver Nanoparticle Decorated Denture Base Polymer.

Abstract

Aim: Biofilm formation and clinical fracture are the most common problems of poly(methylmethacrylate) (PMMA) based denture base materials. This study aimed to investigate the effect of incorporation of PMMA with silver nanoparticles (AgNPs) and evaluation of its mechanical and biologic performance. Materials And Methods: PMMA were incorporated with 1, 2, 5 and 10wt% AgNPs. Various physiochemical characterizations were done using X-Ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and Atomic Force Microscopy (AFM). Mechanical properties were statistically analysed using Post hoc Bonferroni test. Cytotoxic studies were done with MTT assay. Biofilm formation was checked against Candida albicans and Streptococcus mutans. Results: Morphologic analysis revealed uniform distribution of nanoparticle in the matrix. The surface roughness was proportionate with the amount of nanoparticle added. Tensile strength, modulus of elasticity, flexural strength and flexural modulus were improved by the addition of up to 5 wt % of AgNPs with statistical significance. SEM micrographs revealed the fracture resistance of AgNPs by the reduction in the size and direction of cracks. The cytotoxic studies carried out on PMMA/AgNPs revealed good biocompatibility. The composite also showed reduction in the adherence of Streptococcus mutans and Candida albicans. Conclusion: The results indicate that doping PMMA with AgNPs enhances the mechanical properties of the system. The good biocompatibility and anti-biofilm property revealed by this novel system is suitable for various prosthodontic applications such dentures, obturators, ocular prosthesis and cranial prosthesis.