Abstract
In this study, we investigated the trans-membrane dynamics of macromolecules by real-time visualization in on-chip electroporation using field focusing. The device for on-chip electroporation consisted of two parallel microchannels that are connected through a micro-orifice for field focusing and cell trapping. The micro-orifice embedded in a vertical wall of the microchannel was 4.04 μm in width, 3.68 μm in height and 4.0 μm in length. By measuring ionic current through the micro-orifice, we evaluated electrical characteristics of the device. A HeLa cell was trapped at the micro-orifice by aspiration. Then, pulsed voltage, which was 10 V high and 1 ms long, was applied to electrodes located across the micro-orifice. The change of the fluorescence intensities indicated the successful pore formation. By analyzing the time course data of the fluorescence intensity, we discussed the detailed mass transfer through the cell membrane.
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