OS 08-01 ASSOCIATION OF ACE, FABP2, PPARG2, GST, FTO AND CYP46A1 GENES POLYMORPHISM WITH ESSENTIAL HYPERTENSION AMONG NORTH INDIAN POPULATION

Abstract

Objective: Hypertension (HTN) is the most common cardiovascular disease and is assuming epidemic proportions in developing countries. Genetic and environmental factors play important role in the development of hypertension.The objective of this study was to investigate the possible association of ACE, FABP2, PPARG2, GST, FTO and CYP46A1 genes polymorphism and susceptibility of individuals to HTN. Design and Method: This case control study includes 123 essential HTN cases and 102 controls. ACE, GST genes polymorphisms were evaluated by PCR and FABP2, PPARG2, FTO, CYP46A1 genes polymorphisms were evaluated by PCR-RFLP methods. Results: The mean age of cases and controls were 41.32 ± 11.39 years and 40.13 ± 10.28 years. Frequencies of ACE II, ID, DD genotypes in essential HTN cases and controls were 22.76%, 63.42%, 13.82% and 11.77%, 60.78%, 27.45%; respectively. Frequencies of FABP2 AA, AT, TT in cases and controls were 11.38%, 70.74%, 17.88% and 20.59%, 64.71%, 14.70%. Frequencies of PPARG2 CC, CG, GG genotype in cases and control were 27.65%, 65.85%, 6.50% and 21.57%, 37.26%, 41.17%. Frequency of GSTM1 null, GSTT1 null genotypes in cases and controls were 41.47%, 5.69% and 52.95%, 4%; respectively. Frequencies of FTO AA, AT, TT genotype in cases and control were 1.63%, 68.29%, 30.08% and NA, 75.49%, 24.51%. Frequencies of CYP46A1 CC, CT, TT genotype in cases and control were 17.08%, 33.33%, 49.59% and 3.93%, 28.43%, 67.64%. Significant differences were obtained in the frequencies of ACE II genotype (p = 0.032), PPARG2 CG, GG genotype (p < 0.001) CYP46A1 CC, TT genotype (p = 0.002, 0.006). Conclusions: Findings of this study suggest that ACE, PPARG2, CYP46A1 genes were significantly associated with essential HTN cases in North Indian populations.