Oryzihumus terrae sp. nov., isolated from soil and emended description of the genus Oryzihumus

Abstract

A Gram-stain-positive, aerobic, non-flagellated bacterium, designated KIS22-12(T), was isolated from a soil sample of Baengnyeong Island in Onjin county, Republic of Korea. Cells were non-spore-forming cocci showing catalase-positive and oxidase-negative reactions. Growth of strain KIS22-12(T) was observed between 10 and 35 °C (optimum, 28-30 °C), between pH 5.0 and 9.0 (optimum, pH 7.0) and with 0-3% (w/v) NaCl. Strain KIS22-12(T) contained MK-8(H4) as the predominant menaquinone, and C17 : 1ω8c, iso-C15 : 0 and anteiso-C15 : 0 as the major fatty acids. Strain KIS22-12(T) contained diphosphatidylglycerol, phosphatidylinositol, one unknown aminophospholipid, one unknown aminolipid, two unknown phospholipids and one unknown lipid. The peptidoglycan type was A1γ. The G+C content of the genomic DNA was 75.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KIS22-12(T) formed a phyletic lineage with Oryzihumus leptocrescens KV-628(T). 16S rRNA gene sequence similarity between the two strains was 96.5%. On the basis of phenotypic, chemotaxonomic and molecular properties, strain KIS22-12(T) represents a novel species within the genus Oryzihumus, for which the name Oryzihumus terrae sp. nov. is proposed. The type strain is KIS22-12(T) ( = KACC 16543(T) = DSM 27161(T) = NBRC 109596(T)). An emended description of the genus Oryzihumus is also provided.