Orthogonal test for preparing genipin with enzymolyzed geniposide

Abstract

To optimize the process for preparing genipin with enzymolyzed geniposide by an orthogonal experiment. The optimal enzymolysis process was selected by an orthogonal experiment, with the concentration of geniposide as the index as well as enzyme quantity, pH of enzymolysis solution, enzymolysis time and enzymolysis temperature as considerations. The optimal hydrolysis conditions were as follow: rough genipin samples at the concentration of 40 g x L(-1) were selected and shook on a table concentrator at a speed of 100 r x min(-1), added with beta-glucosidase-geniposide 1 : 1 (weight proportion), with pH of enzymolysis solution at 4.5, hydrolyzation temperature at 50 degrees C, the conversion rate of genipin could reach 85.8%. The process is so stable and feasible that it can provide theoretical basis for the preparation of genipin with enzymolyzed geniposide.