Abstract
PEG-induced fusion of two haploid Saccharomyces strains resulted in three morphologically different types of fusion products. In order to estimate the respective ploidy, one representative of each type — F1, F2 and F3 — was subjected to a measurement of the cellular DNA content and to a meiotic segregation analysis. The data obtained in these analyses suggested the strain F1 to be a haploid cybrid resulting from mere plasmogamy, whereas the hybrids F2 and F3 were likely to be the products of a successful nuclear fusion of two, respectively three, protoplasts of the parental strains. However, the complete genetic composition of the hybrids could only be revealed by OFAGE experiments, as the genetic data solely referred to a few chromosomes with marker genes. All the results of the OFAGE were in full accordance with the assumptions made in the conventional analysis, thus indicating the OFAGE to become a very promising means for the investigation of hybrids inaccessible to genetic analyses.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
