Orthogonal-Field-Alternation Gel Electrophoresis Banding Patterns of Some Asporogenous Yeasts and their Respective Ascosporogenous States

Abstract

Summary The relationship of seven Candida species and their corresponding ascogenous perfect states was studied by orthogonal-field-alternation gel electrophoresis (OFAGE). The following pairs produced similar OFAGE-patterns: C. lipolytica and Yarrowia lipolytica, C. pelliculosa and Hansenula anomala, C. pseudotropicalis and Kluyveromyces marxianus var. marcianus, C. utilis and Hansenula jadinii and C. shetatae and Pichia stipitis . There was no apparent relationship between C. parapsilosis and Lodderomyces elongisporus as well as C. kefyr and Kluyveromyces marxianus var. marxianus . The close correlation found between the OFAGE patterns of these yeasts indicate that electrophoretic karyotyping may be useful for studying relationships between perfect and imperfect yeasts.