Abstract
Flavonoids are bioactive constituents in Oroxylum indicum seeds, an Asian traditional remedy used for the treatment of respiratory infections. In this study the first capillary electrophoretic method for their determination is presented. By using a 25 mM borax buffer at pH 9.2 containing 10 mM SDS as detergent, the determination of seven flavonoids was feasible in only 13 min. Method validation confirmed that the assay is in accordance with ICH requirements in respect to linearity, selectivity, sensitivity, accuracy and precision. Quantitative results revealed that baicalein-7-O-gentiobioside is the most abundant flavonoid in the drug (1.19 to 5.33%), followed by other baicalein derivatives (7-O-glucoside, 7-O-glucuronide). These observations were in good qualitative and quantitative agreement with LC-MS results.
Highlights
Oroxylum indicum (L.) Vent. (Bignoniaceae) is an up to 12 m high tree, native to the Indian subcontinent (Assam to southern China)
Termed as mùhúdié a monograph on O. indicum seeds can be found in the current version of the Chinese Pharmacopeia [3]
One of the limitations of capillary electrophoresis (CE) is the fact that under ―normal‖ conditions only charged analytes can be separated; neutral compounds will migrate unresolved with the electroosmotic flow (EOF)
Summary
Oroxylum indicum (L.) Vent. (Bignoniaceae) is an up to 12 m high tree, native to the Indian subcontinent (Assam to southern China). Termed as mùhúdié a monograph on O. indicum seeds can be found in the current version of the Chinese Pharmacopeia [3]. Pharmacological studies confirmed anti-inflammatory and anti-oxidative activities of the respective extracts and especially emphasized on the role of flavonoids as bioactive constituents [4,5,6]. These compounds (mainly baicalein and chrysin glycosides) are considered as indicators for drug quality and efficacy
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