Orosomucoid 1 Expression In Peripheral Blood Mononuclear Cells Following Resistance Exercise

Abstract

An acute bout of resistance exercise (RE) can produce inflammation and subsequent changes in immune cells. Limited numbers of gene transcripts related to the immune and inflammatory processes have been examined. Orosomucoid 1 (ORM1) is an acute phase plasma protein that may be involved in immunosuppression. DNA microarrays have been utilized to examine exercise effects on gene expression in peripheral mononuclear cells (PBMCs). The present study utilized an acute bout of RE to examine the expression of inflammatory genes in PBMCs. Ten males performed an acute bout of RE. Venous blood was sampled at rest, post-exercise, and at 2 hr post-exercise, and was analyzed for gene expression using Affymetrix Genechip Arrays. Using a conservative false discovery rate of p<0.05 and a two-fold change of or higher threshold, microarray analysis identified 167 genes differently expressed between baseline and 2 hr post-exercise, 259 genes between 2 hr post-exercise and immediately post-exercise, and six genes between immediately post-exercise and baseline indicating the greatest gene response was seen at the 2 hour post-exercise. At the 2 hr time point, ORM1 showed significant up regulation. In conclusion, RE is capable of influencing gene expression in numerous inflammatory mediators. Induction of ORM1 suggests that it may play an important role in immune responses following RE. Supported by the Vermont Genetics Network.