Orodispersible Film Loaded with Enterococcus faecium CRL183 Presents Anti-Candida albicans Biofilm Activity In Vitro.

Virgínia Barreto Lordello,Andréia Bagliotti Meneguin,Sarah Raquel De Annunzio,Maria Pía Taranto,Daniela Cardoso Umbelino Cavallini,Carla Raquel Fontana,Marlus Chorilli

doi:10.3390/pharmaceutics13070998

Virgínia Barreto Lordello, Andréia Bagliotti Meneguin + Show 5 more

Open Access

https://doi.org/10.3390/pharmaceutics13070998

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Journal: Pharmaceutics	Publication Date: Jun 30, 2021
Citations: 12	License type: CC BY 4.0

Affiliation: Universidade Estadual Paulista (Unesp)

Abstract

Background: Probiotic bacteria have been emerging as a trustworthy choice for the prevention and treatment of Candida spp. infections. This study aimed to develop and characterize an orodispersible film (ODF) for delivering the potentially probiotic Enterococcus faecium CRL 183 into the oral cavity, evaluating its in vitro antifungal activity against Candida albicans. Methods and Results: The ODF was composed by carboxymethylcellulose, gelatin, and potato starch, and its physical, chemical, and mechanical properties were studied. The probiotic resistance and viability during processing and storage were evaluated as well as its in vitro antifungal activity against C. albicans. The ODFs were thin, resistant, and flexible, with neutral pH and microbiologically safe. The probiotic resisted the ODF obtaining process, demonstrating high viability (>9 log10 CFU·g−1), up to 90 days of storage at room temperature. The Probiotic Film promoted 68.9% of reduction in fungal early biofilm and 91.2% in its mature biofilm compared to the group stimulated with the control film. Those results were confirmed through SEM images. Conclusion: The probiotic ODF developed is a promising strategy to prevent oral candidiasis, since it permits the local probiotic delivery, which in turn was able to reduce C. albicans biofilm formation.

Highlights

It is well established that probiotics, which are defined as “living microorganisms that, when administered in adequate amount, confer a health benefits to the host” [1], can improve dysbiotic microbiota, regulate intestinal transit, neutralize carcinogens, promote vitamin synthesis and bile salt metabolism, reinforce gut barrier and the immune system, and directly antagonize and compete with pathogens [2]
Considering the above, this study aimed to develop and characterize an orodispersible film (ODF) with the addition of the probiotic E. faecium CRL 183, evaluating its antifungal potential against C. albicans ATCC
The probiotic strains E. faecium CRL 183 and C. albicans ATCC 90028 were obtained from the Reference Center for Lactobacilli—CERELA/CONICET (San Miguel de Tucumán, Argentina) and from American Type Culture Collection (Manassas, VA, USA), respectively

Summary

Introduction

It is well established that probiotics, which are defined as “living microorganisms that, when administered in adequate amount, confer a health benefits to the host” [1], can improve dysbiotic microbiota, regulate intestinal transit, neutralize carcinogens, promote vitamin synthesis and bile salt metabolism, reinforce gut barrier and the immune system, and directly antagonize and compete with pathogens [2]. Several studies have shown that specific probiotic strains have great potential for improving oral health, presenting anticariogenic activity, reduction of halitosis, and prevention of opportunistic infections, such as candidiasis, which are often present in immunocompromised individuals [3,4,5,6,7]. These pathogenic conditions are related to the formation and establishment of biofilms in the oral cavity.

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