Ornithinibacillus composti sp. nov., isolated from sludge compost and emended description of the genus Ornithinibacillus.

Abstract

A Gram-stain positive, aerobic, motile, endospore-forming and rod-shaped bacterium, designated GSS05(T), was isolated from a sludge compost sample and was characterized by means of a polyphasic taxonomic approach. Growth was observed to occur with 0-3% (w/v) NaCl (optimum 1%), at pH 5.5-10 (optimum pH 7.5) and at 15-50°C (optimum 37°C). According to the results of a phylogenetic analysis, strain GSS05(T) was found to belong to the genus Ornithinibacillus and to be related most closely to the type strains of Ornithinibacillus halotolerans and Ornithinibacillus contaminans (96.5 and 95.1% 16S rRNA gene sequence similarity, respectively). The peptidoglycan amino acid type was determined to be A4β. The major respiratory quinone was identified as menaquinone-7 (MK-7). The polar lipid profile of strain GSS05(T) was found to contain a predominance of diphosphatidylglycerol, moderate amounts of phosphatidylglycerol and minor amounts of two unknown phospholipids and two unknown lipids. The G+C content of genomic DNA was determined to be 42.1mol%. The dominant cellular fatty acids were identified as iso-C15:0 and anteiso-C15:0. The phenotypic, chemotaxonomic, phylogenetic and genotypic data indicated that strain GSS05(T) represents a novel species of the genus Ornithinibacillus, for which the name Ornithinibacillus composti sp. nov. is proposed. The type strain is GSS05(T) (=CCTCC AB 2013261(T)=KCTC 33192(T)).