Abstract
A simple and direct method for the detection of ornithine decarboxylase (EC 4.1.1.17) activity is presented. It is based upon the selective binding of putrescine to Whatman P81 paper, a strong cation-exchanger, in the presence of 0.1 m NH 3. The assay is easy to perform and has an advantage over the more frequently used CO 2 trapping methods which can yield spurious CO 2 formation due to the action of enzymes other than ornithine decarboxylase.
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