Abstract
The monitoring of the chromatographic resolution for three pairs of analytes separated in different chromatographic systems using mobile phases with varying concentrations of viscous fast atom bombardment matrices showed that it exhibits a steady decrease with increasing matrix content in the mobile phase. The decrease in resolution is observed in the partition and ion-pair chromatographic modes at both low and high matrix contents in the eluent for both conventional and capillary chromatographic systems using precolumn addition of glycerol and thioglycerol. Careful examination of the normalized efficiency, capacity factor and selectivity terms contributing to the resolution allowed the identification of the sources of the decrease in resolution in the presence of a matrix in the eluent. Ale efficiency and capacity factor terms show decreases with increasing matrix content in all systems. Subtle variations in the selectivity term observed in the presence of a viscous matrix can increase or limit the decrease in resolution. The variations observed for the efficiency term show similar trends in all systems studied and appear to be independent of the analyte or the chromatographic mode. However, the variations in capacity factor and selectivity induced by the presence of the viscous matrix are dependent on the nature of the analyte, the type of chromatography and the nature of the matrix.
Published Version
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