Origin of small primary afferent substance P-immunoreactive nerve fibers in the guinea-pig

Abstract

Primary afferent and substance P (SP)-immunoreactive nerve fibers of the guinea pig and rat heart were investigated by physiological and immunohistochemical methods. Immunohistochemistry revealed abundant SP-positive fibers in the guinea pig atria, with fewer in the ventricles. Only an occasional fiber was seen in the rat atrium or ventricle. Sectioning the vagus nerve did not noticeably influence the supply of SP-immunoreactive nerve fibers in the guinea pig heart. When the atria or ventricles were stimulated, afferent nerve fiber activity was recorded from the second and third thoracic dorsal roots. In guinea pig atria 3 types of fibers were identified on the basis of conduction velocities: A δ 1, A δ 2 and C fibers. Only Aδ fibers were identified in the ventricle. By vagal recordings, Aδ fibers were demonstrated but a C fiber response could not be shown in this nerve. SP-immunoreactivity in primary afferent fibers was depleted by the neurotoxin capsaicin. Capsaicin treatment also caused a reduction in the conduction velocity of small diameter myelinated A δ 2 (by 29%) and unmyelinated C fibers (by 46%). In the rat heart, evidence for A δ 2 or C fibers was not found. These results indicate that primary afferent and SP-immunoreactive fibers are numerous in guinea pig heart, but few in the rat. It is concluded that most of these fibers have their cell bodies of origin in the dorsal root ganglia.