Abstract
On isoelectric focusing, bakers yeast enolase A has been shown to resolve into one major and at least two minor species. Refocusing of individual species isolated by electrofocusing shows the minor species to be formed from the major one. The extent of formation of the minor species increases with electrofocusing time and decreasing quantity of protein electrofocused, suggesting that the protein, on dissociating, tends to change into forms(s) with more alkaline isoelectric points.
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