Origin Analysis of Resistance Gene to Soybean Mosaic Virus in Soybean Line ICGR95-5117

Abstract

Soybean mosaic virus (SMV) is a prevalent viral pathogen of soybean. The soybean mosaic virus N3 (SMV3) is the most toxic strain in northeast China. Soybean lines ICGR95-5117 and ICGR95-5383 with high resistance to SMV3,which were all derived from ICGR661,are recently released from Chinese Academy of Agricultural Sciences. Several genes resistant to soybean mosaic virus have been identified and mapped on different soybean linkage groups by molecular markers. The re-sistance gene in ICGR95-5383 has been mapped on soybean linkage group F near Rsv1 gene. A dominant marker developed from the candidate gene of Rsv1 has been reported recently. The purpose of this research was to find the origin of the SMV3 resistance gene in ICGR95-5117 and the relationship of this resistance gene with Rsv1 by analyzing the pedigree. Thirty four SSR markers selected from linkage group F and two markers,SCAR marker (SCN11) tightly linked with resistance gene in ICGR95-5383 and Rsv1-f/r makers co-segregated with resistance gene Rsv1,were used to detect the materials in pedigree of ICGR95-5117. The similarity coefficient of the two lines between ICGR95-5117,ICGR95-5383 and ICGR661 was the highest. All the material except for Mangnolid(F-53)B had SMV3 resistance allele at locus SCN11. Williams 82 and ICGR661 had the same allelic variation at Rsv1-f/r locus with ICGR95-5117,revealing that the Rsv1 gene in ICGR95-5117 was inherited from Williams 82 via ICGR661. But ICGR95-5117 had higher resistance than ICGR661,indicating it might inherit different genes from SMV3 resistance parent Ludou4.