Origanum onites ve Mentha spicata subs. tomentosa Uçucu Yağ Nanoemülsiyonlarının Bakteriyel Balık Patojenlerine Karşı in Vitro Antibakteriyel Aktivitesi

Abstract

The current study aimed to determine the antimicrobial activities of two different aromatic plants (Origanum onites, Mentha spicata subs. tomentosa) essential oils, and their nanoemulsion formulations against six common fish pathogens, Pseudomonas aeruginosa, Vibrio parahaemolyticus, Aeromonas veronii, Vibrio alginolyticus, Yersinia ruckeri, and Lactococcus garvieae. The main components of Mentha spicata subs. tomentosa essential oil (MEO) were piperitone (25.01%), eucalyptol (1,8-cineole) (19.53%), pulegone (14.50%) and, Piperitenone (10.98%). The major components of Origanum onites essential oil (OEO) which were carvacrol (46.17%) and, p-cymene (13.05%) were detected. The antibacterial effects of OEO and MEO and their nanoemulsions were determined by using the agar disc diffusion method. The OEO and its nanoemulsions were extremely effective against the Gram‐negative Aeromonas veronii than the positive control (enrofloxacin). In addition, it was observed that OEO nanoemulsion was more effective than OEO in terms of antibacterial activity. MEO and its nanoemulsions were found to have low activity against fish pathogens, however, there was no activity on Aeromonas veronii and Lactococcus garvieae.