Oriented Conjugates of Single-domain Antibodies and Fluorescent Quantum Dots for Highly Sensitive Detection of Tumor-associated Biomarkers in Cells and Tissues

Abstract

Our recent results in the field of engineering and application of highly oriented conjugates of single-domain antibodies and fluorescent quantum dots are summarized. These novel conjugates proved to be excellent nanoprobes for immunolabeling of tumor-associated biomarkers on cells and tissue specimens detectable by means of flow cytometry or one- or two-photon confocal microscopy. The results are discussed in terms of the most promising future applications of these conjugates and further developments in this field.