Abstract
Avidin molecules were immobilized on a COOH-modified SiO 2/Si(1 0 0) surface with subnano-level flatness (root-mean-square (rms) roughness ≈0.1 nm) forming covalent bonds between the COOH groups on the substrate surface and the NH 2 groups of avidin molecules. The structures of avidin-immobilized surfaces were investigated by atomic force microscopy (AFM), ellipsometry, and infrared (IR) reflection absorption spectroscopy using buried metal layer substrate (BML-IRRAS), and transmission IR absorption spectroscopy (TIRAS). These data have evidenced that the avidin molecules are immobilized with the 2-fold symmetry axis of the tetramer almost perpendicularly to the substrate surface.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
