Oridonin inhibits breast cancer cell proliferation and migrationthrough suppressing COX-2 expression

Abstract

Oridonin, a biologically active substance isolated from the plant Rabdosia rubescens, is recently reported to have aninhibitory effect on breast cancer. However, its mechanism is still unclear. According to previous findings, increasedCOX-2 expression has been associated with a higher incidence of breast cancer. Therefore, this study aims to explorewhether oridonin inhibits cell proliferation and migration by suppressing the expression of COX-2 in human breastcancer cells MDA-MB-231 in vitro and in vivo conditions. In this study, human breast cancer cells MDA-MB-231will be treated with increasing concentrations and for various durations with oridonin to investigate whether oridonininhibits cell migration, decrease proliferation, and reduce tumors in mice through suppressing the expression of COX2. A positive control is a taxol. A negative control is medium. Cell migration will be tested by Transwell assay. Theproliferation will be detected by MTT assay. The western-blotting assay will be used for COX-2. In nude mice, the invivo anti-tumor efficacy of oridonin will be examined. The study’s result will provide insight into the effect of oridoninon the proliferation and migration of human breast cancer cells MDA-MB-231. It will also provide reference for themechanism of how oridonin inhibits breast cancer and lay the foundation for future studies on the molecular basis oforidonin’s pharmacological activity in the future.