Organosolvent Extraction Coupled with Steam Explosion Pretreatment of Wild Sugarcane and Optimization of Cellulase Activity using Response Surface Methodology

S Aruna,Sriramulu Gobikrishnan

doi:10.22207/jpam.15.1.06

Abstract

The present study focuses on the effect of the organosolvent extraction with steam explosion pretreatment and optimization of cellulase activity using the response surface methodology for the production of bioethanol. The commercial cellulase enzyme was characterized for its optimal pH, temperature, and also studied for the enzyme activity. The effect of organosolvent pretreatment was identified in both native and pretreated wild sugarcane by SEM, XRD, and FTIR. The cellulase enzyme was found to be tolerant with an optimal pH of 5.0 with cellulase activity of pretreated wild sugarcane is found to be 25.36 IU/ml. Organosolvent extraction of wild sugarcane was carried out using water and ethanol as solvent. The composition of lignin, hemicelluloses, and cellulose was also determined in both pretreated and native biomass. The activity of the enzyme was observed over the effect of enzyme loading, substrate loading, temperature, and pH using a Box-Behnken design.

Highlights

Bioethanol is ecofriendly since it is produced from renewable energy sources of biomass which can be utilized as an alternative to fossil fuel[1]
The present study focuses on the effect of the organosolvent extraction with steam explosion pretreatment and optimization of cellulase activity using the response surface methodology for the production of bioethanol
The results indicated that organosolvent extraction with steam explosion pretreatment increased the yield of cellulose than to native wild sugarcane shown in Table

Summary

Introduction

Bioethanol is ecofriendly since it is produced from renewable energy sources of biomass which can be utilized as an alternative to fossil fuel[1]. Plant biomass consists of carbohydrates such as cellulose, hemicelluloses, starch, and pectin which can be a suitable substrate for biofuel production[5]. The method explodes biomass by rapid depressurization from extreme temperature and pressure situations to increase the responsiveness of cell wall content to cellulase thermal decomposition to achieve higher yields for subsequent fermentation. Steam explosion is a thermo-hydrolytic pretreatment process for enhancing enzymatic saccharification and synthesis of bioethanol[7]. 8-13 Organosolvent pretreatment helps to increase the yield of conversion during fermentation by influencing the acid, enzymatic, and microbial action[14]. For the production of bioethanol by organosolvent pretreatment the wild sugarcane was examined. The current study was aimed to know the efficiency of organosolvent extraction with steam explosion pretreatment to induce the structural and chemical changes made by thermophysical pre-treatment in the wild sugarcane

Methods

Results

Conclusion