Organogenesis on Root, Leaf, Stem-Disc, and Scape Explants of Eryngium foetidum L., a Rare Medicinal Plant

Abstract

ABSTRACT A single medium-based plant regeneration protocol via organogenesis from root, leaf, stem-disc, and scape explants of Eryngium foetidum L. was established on Murashige and Skoog (MS) medium supplemented with growth regulators. Shoot and root induction on media prepared with tap water and commercial sugar did not differ from that prepared with double-distilled water and tissue culture grade sucrose. Both the type of explant tissue and the growth regulator added to the media significantly influenced the number of shoots. Kinetin was more effective than benzyladenine in inducing shoot formation. A combination of 4.65 μM Kn and 2.22 μM BA induced the greatest number of shoots on all types of explants. Scape explants were the best for shoot formation and developed a mean of 37.2 shoots per explant on the combination of Kn and BA. Callus formation was a characteristic of the BA supplemented medium. Shoot initiation/multiplication media (MS with 4.65 μMKn and 2.22 μMBA) facilitated development of roots from the base of 70 percent of the shoots. Rootless shoots transferred onto half-strength MS basal medium developed a mean of 16.2 roots per shoot. Upon transfer to the field, 90 percent of the plantlets survived and exhibited morphological characters identical to those of the source plant.