Organogenesis in vitro from the leaf of blackberry cv Cacanska bestrna

Abstract

The paper presents evaluation of different types and concentrations of plant growth regulators on induction of adventitious organogenesis from the leaf of blackberry cultivar Cacanska Bestrna. This genotype was introduced and maintained in tissue culture on Murashige and Skoog medium (MS) (1962), mineral salts and organic complex with in mg l-1: BAP 1.0, IBA 0.1, GA3 0.1. Young, expanded leaves taken from upper third of in vitro proliferating shoots were used as initial explants. The leaves were cut three times across the midrib and placed with adaxial side touching the regeneration medium. In this experiment 20 types of regeneration media have been evaluated with different types and concentrations of cytokinins and auxins: BAP (2.0 mg l-1) or TDZ (1.0 mg l-1) alone, or each in combination with different concentrations of IBA, NAA and 2,4-D (0.1, 1.0 and 2.0 mg l-1). In cv Cacanska Bestrna regeneration of adventitious shoots was observed on three different types of media with BAP combined with IBA (0.1 and 1.0 mg l-1) and NAA (0.1 mg l-1). TDZ alone or in combination with IBA (0.1, 1.0 and 2.0 mg l-1) and NAA (0.1 mg l-1) has been more effective at induction of regeneration than BAP. The highest percentage of regeneration was obtained precisely on medium supplemented with TDZ alone (41.66%). .