Abstract
Summary A comparison of the soluble, membrane- and cell wall-bound peroxidases of an organogenetic and a nonorganogenetic habituated sugarbeet callus showed that organogenetic tissues contained a higher level of peroxidase activity and a larger number of peroxidase isoenzymes. Partially (through G-100 Sephadex) purified peroxidases of organogenetic callus likewise exhibited higher auxin-oxidase activities. Organogenetic tissues also were found to contain higher levels of peroxidase inhibitors and auxin protectors (auxin-oxidase inhibitors). A GC-MS analysis of IAA indicated a lower level of the hormone in the organogenetic callus. This presumably leads to an auxin-cytokinin ratio compatible with the induction of shoot formation.
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