Organogenesis in Cultured Pinus sylvestris Tissue

Abstract

Summary Coumarin (10 µM), alone or in combination with IAA and BAP, induced a relatively high rooting-frequency in plumule explants of 14-day-old Pinus sylvestris seedlings; its effect on needle explants was less marked and erratic. Adventitious buds were readily induced in needle explants that consisted of the entire short shoot or dwarf spur, the retention of the growth-arrested shoot apex seemingly having an enhancing effect. Needle explants without the dwarf spur produced prolific callus but were very much less responsive organogenically.