Abstract
Organogenesis induction from internodal segments of yellow passionfruit (Passiflora edulis f. flavicarpa) was obtained in a medium supplemented with 1.0-4.0 mg L-1 benzylaminopurine (BAP). Callus proliferation and adventitious shoot development were analyzed by light and scanning electron microscopy. Callus proliferation and adventitious shoots were observed simultaneously and continuously, with asynchronous development of shoots in the explants. Proliferation of callus was observed in the explant cut ends in all treatments, including the control. The results show that concentrations of 1.0 or 2.0 mg L-1 BAP gave better rate of organogenesis. Higher concentrations were efficient for the induction of adventitious buds and, were, however, detrimental for subsequent shoot development. Growth and rooting of shoots were obtained in half strength medium without growth regulators.
Highlights
Passiflora edulis f. flavicarpa, yellow passionfruit, is a tropical fruit, native to Brazil and widely distributed throughout the tropics and subtropics
Tissue Culture: Seeds of yellow passionfruit were germinated in the greenhouse
Light Microscopy (LM): Samples were fixed in a solution of freshly prepared paraformaldehyde (3% w/v) and glutaraldehyde (2% v/v) in 0.2 M cacodylate buffer
Summary
Passiflora edulis f. flavicarpa, yellow passionfruit, is a tropical fruit, native to Brazil and widely distributed throughout the tropics and subtropics. Flavicarpa, yellow passionfruit, is a tropical fruit, native to Brazil and widely distributed throughout the tropics and subtropics. An acidic juice is obtained and used for juices, sherbets, jams and jellies (Purseglove, 1968). Leaves of this species contain alkaloids with potential use for the pharmaceutical industry (Lotschert & Beese, 1989). It is a perennial plant that can be propagated by seeds, cuttings, air-layering, or grafting. Commercial areas, are based on seedlings, which generate high levels of genetic variability (Drew, 1997). In vitro culture can be useful for clonal propagation of superior genotypes and disease resistant rootstocks, and as a tool for genetic transformation
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