Abstract
The production of potatoes rich in starch and essential nutrients does not cover the needs of Congolese consumers. This is due to strong genetic erosion and the absence of high-performance local varieties as well as the unavailability of seeds for new plantations. The seed produced by traditional techniques does not meet the demand of producers. This study aims to produce seed potatoes by the technique of in vitro culture. Tubers disinfected with 10% sodium hypochlorite are kept at 4°C for 20 days to produce microtubers by bud burst others are placed in heat therapy conditions to produce micro-cuttings. Microtubers and microcuttings are taken, then cultured in vitro on Murashige and Skoog medium on the one hand and on Murashige and Skoog medium to which 0.05 mg/ml of indolylbutyric acid is added on the other hand. The cold favors the bud burst of the potato tubers after a week. On vitroplants from microtubers developing on Murashige and Skoog medium to which 0.05 mg / ml of indolylbutyric acid is added, 5 roots, 6 leaves and a stem with a height of 6.05 cm are recorded. On the medium of Murashige and Skoog, we observe 3 roots, 7 leaves and a stem 5.69 cm in height. Regarding microprogation, the results obtained showed that growth hormone plays an important role in the different stages of regeneration compared to the medium without phytohormone.
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