Organochlorine pesticides and polychlorinated biphenyls in European roe deer Capreolus capreolus resident in a protected area in Northern Italy

Abstract

In 2001, samples of liver ( n=38) and perirenal fat ( n=25) (only in males) were taken from European roe deer ( Capreolus capreolus) populating a hilly, wooded protected area in the Emilia-Romagna region of Northern Italy. Samples were analyzed for organochlorine pesticides dichlorodiphenyltrichloroethane (DDT) and its metabolites, hexachlorobenzene (HCB), hexachlorocycloexane α-, β- and γ-isomers (α-, β- and γ-HCH), α-endosulfan, aldrin, dieldrin and endrin, and the seven PCB ‘indicator’ congeners IUPAC Nos. 28, 52, 101, 118, 138, 153 and 180, in order to investigate the current state of contamination of the area and to assess the possible toxicological risks for the monitored species. p,p′-DDE was detected in 95% of the liver and perirenal fat samples ( n=63), at concentrations ranging from <dl to 629.3 ng/g lipid weight, which were much higher than those found for HCB and γ-HCH. The concentrations of the other measured organochlorine pesticides and metabolites were below the detection limit in all the samples. PCBs were found in 97% of the liver and perirenal fat samples, at total levels ranging between <dl to 414.5 ng/g lipid weight. The hepta- and hexa-chlorinated congeners 180, 153 and 138 were predominant and comprised more than 70% of the total seven PCBs in both the liver and perirenal fat samples. In the male roe deer, significantly higher concentrations of HCB, p,p′-DDE and PCBs were found in the liver than in the perirenal fat ( P<0.001). Hepatic levels of p,p′-DDE and PCBs were significantly higher in males than in females ( P<0.05 and P<0.001, respectively). No significant differences in organochlorine concentrations were observed between juvenile and adult males in either tissue sample ( P>0.05). Females were not statistically tested because they were all adults. The levels of organochlorine contaminants were well below those associated with adverse reproductive effects and lethality in mammals.