Organization of WAVE-1 in hippocampal dendritic spines

Abstract

Event Abstract Back to Event Organization of WAVE-1 in hippocampal dendritic spines R. Szudóczky1, R. Weinberg2, S. Soderling3, P. Sótonyi1 and B. Rácz1* 1 St. István University, Dept. Anatomy & Histology, Hungary 2 University of North Carolina, Dept. Cell & Developmental Biology, United States 3 Duke University Medical Center, Dept. Cell Biology, United States Recent in vitro studies have shown that theWAVE (Wiskott–Aldrich syndrome Verprolin-homology) family of actin binding proteins play an important role in modulating synaptic plasticity. Moreover, dysregulation of WAVEs has been implicated in a syndromic form of mental retardation in humans, reproduced in WAVE-1 knockout (KO) mice. Loss of WAVE-1 leads to abnormalities in synaptic plasticity and deficits in memory retention, suggesting that WAVE-1 plays a key role in spine functions associated with synaptic plasticity. Here we studied EM sections from WT and WAVE-1 KO mice. Spine dimensions did not differ significantly between WT and KO, but we found significantly more trafficking endosomes in spines from KO mice than in WT controls. We also studied the organization of WAVE-1 in hippocampus. LM immunostaining revealed WAVE-1 in dendrites of pyramidal neurons; high-resolution EM labeling techniques showed that WAVE-1 extended into dendritic spines. Quantitative analysis of the gold label confirmed a close association of WAVE-1 with the spine plasma membrane. Density was especially high in the so-called “endocytic zone.” We hypothesize that WAVE-1 plays a pivotal role in the organization of the actin cytoskeleton necessary for intraspinoplasmic vesicle trafficking. Keywords: Neurophysiology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Neurophysiology Citation: Szudóczky R, Weinberg R, Soderling S, Sótonyi P and Rácz B (2011). Organization of WAVE-1 in hippocampal dendritic spines. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00050 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. B. Rácz, St. István University, Dept. Anatomy & Histology, Budapest, Hungary, racz.bence@aotk.szie.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers R. Szudóczky R. Weinberg S. Soderling P. Sótonyi B. Rácz Google R. Szudóczky R. Weinberg S. Soderling P. Sótonyi B. Rácz Google Scholar R. Szudóczky R. Weinberg S. Soderling P. Sótonyi B. Rácz PubMed R. Szudóczky R. Weinberg S. Soderling P. Sótonyi B. Rácz Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.