Organisms Recovered from Cases of Canine Pyoderma and their Antibiogram Pattern

Abstract

Canine pyoderma is one of the most common causes of dermatitis with worldwide occurrence in small animal practice. The condition is diagnosed on the basis of clinical manifestations, isolation and identification of causative organisms by bacteriological cultural examination. A study on 130 clinical cases of canine pyoderma was conducted at the Teaching Veterinary Clinical Complex (TVCC), Veterinary College, Anand during July, 2016 to April, 2017. In the study undertaken, bacteriological culture examination of 116 pus swabs resulted in the recovery of 165 bacterial isolates. Exudate/pus samples were collected and subjected to bacteriological cultural isolation, identification and subsequently in vitro antibiotic sensitivity testing. On culture, staphylococci were the most predominantly isolated organisms. Amongst staphylococci, Staphylococcus pseudintermedius (49.69%, n=82), a coagulase-positive staphylococci (CPS), was the most predominantly isolated organism, followed by Staphylococcus aureus (18.18%, n=30). Moreover, coagulase-negative staphylococci (CNS), Staphylococcus epidermidis (3.03%, n=5) and S. saprophyticus (0.60%, n=1) were also recovered. The methicillin-resistant staphylococci accounted for 40.07% of the total isolates. Gram negative organisms like Pseudomonas aeruginosa (15.15%, n=25), Klebsiella pneumoniae (12.12%, n=20) and Escherichia coli (0.60%, n=1); and a lone isolate of Streptococcus spp. (0.60%) were also isolated majorly in the form of mixed infections. When subjected to in vitro antibiotic sensitivity testing the isolates showed highest sensitivity to linezolid followed by enrofloxacin, cephadroxil, clindamycin and amoxicillin-clavulanic acid whereas resistance was exhibited against erythromycin, methicillin and oxacillin.