Abstract
Physarum polycephalum nucleolar satellite DNA has been analysed by restriction enzyme digests and hybridisation to ribosomal RNA. The nucleolar DNA is isolated as molecules of molecular weight 39 X 10(6). The positions of sites of endo R-EcoRI and endo R-HindIII digestion have been determined accurately in the whole molecule. The endo R-EcoRI sites and one endo R-HindIII site are within the26-S rRNA complementary sequence and the other endo R-HindIII site is within the 19-S rRNA complementary sequence. The sites are arranged symmetrically about the centre of the molecule as if it were a palindrome.
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