Organic semiconductor self-powered photoelectrochemical strategy for L. Monocytogenes detection coupled with target-triggered DNA polymerase activity

Abstract

The materials involved in studies of self-powered modes are mainly focused on inorganic semiconductor, heterostructure and homojunction. Here, it was found that the self-powered characteristics can be realized based on the photocurrent of polymer organic semiconductor, amino-modified 3,4,9,10-perylenetetracarboxylic dianhydride polymer (PTC-NH2), which being mediated with methylene blue (Mb) and ascorbic acid (AA). A self-powered organic semiconductor photoelectrochemical (PEC) strategy based on co-mediation of Mb and AA was developed for L. monocytogenes detection. This PEC sensing strategy used the target to activate the activity of Taq DNA polymerase. The activated Taq DNA polymerase hydrolysis the DNA which being labeled with Mb. Thereby detaching Mb from magnetic beads (MBs), the strong photocurrent at 0 V with the combined action of AA was produced from PTC-NH2 modified ITO, and realize target detection sensitively. The limit of detection was 85 CFU/mL of L. monocytogenes. This research provides a promising application for the detection of target with the organic semiconductor self-powered PEC sensing strategy. It is anticipated this organic semiconductor self-powered method can give a promising approach for the design and development of low-power-consumption electronics and flexible wearable detection equipment.