Abstract
Real-time monitoring of vessel dysfunction is of great significance in preclinical research. Optical bioimaging in the second near-infrared (NIR-II) window provides advantages including high resolution and fast feedback. However, the reported molecular dyes are hampered by limited blood circulation time (~ 5–60 min) and short absorption and emission wavelength, which impede the accurate long-term monitoring. Here, we report a NIR-II molecule (LZ-1105) with absorption and emission beyond 1000 nm. Thanks to the long blood circulation time (half-life of 3.2 h), the fluorophore is used for continuous real-time monitoring of dynamic vascular processes, including ischemic reperfusion in hindlimbs, thrombolysis in carotid artery and opening and recovery of the blood brain barrier (BBB). LZ-1105 provides an approach for researchers to assess vessel dysfunction due to the long excitation and emission wavelength and long-term blood circulation properties.
Highlights
Real-time monitoring of vessel dysfunction is of great significance in preclinical research
The optical imaging penetration depth is limited to several centimeters, continuously monitoring dynamic physical process of small animals would be helpful to broaden the understanding of vascular dysfunction and recovery process[5]
LZ-1105 administration outlined the cerebral vascular structures clearly at a depth over 1.3 mm under the intact scalp and skull (Fig. 5b and Supplementary Fig. 26). These results suggested that LZ-1105-based long-term NIR-II imaging could facilitate realtime tracking of the temporary opening and recovery of the blood brain barrier (BBB) in the mouse brain, providing an effective method to monitor the pathogenesis of brain diseases, and investigate the drug delivery and treatment strategies
Summary
Real-time monitoring of vessel dysfunction is of great significance in preclinical research. The reported molecular dyes are hampered by limited blood circulation time (~ 5–60 min) and short absorption and emission wavelength, which impede the accurate long-term monitoring. Thanks to the long blood circulation time (half-life of 3.2 h), the fluorophore is used for continuous real-time monitoring of dynamic vascular processes, including ischemic reperfusion in hindlimbs, thrombolysis in carotid artery and opening and recovery of the blood brain barrier (BBB). We report the small molecule LZ-1105 as a NIR-II probe for long-term in vivo imaging of dynamic vascular structure changes in small animals. Pharmacokinetics of LZ-1105 demonstrates a half-life of over 3 h in blood circulation, which provides a long imaging window for long-term monitoring of dynamic vascular structure changes. NIR-II imaging with the long-term angiography probe LZ-1105 in small animals might help researchers to better understand dynamic vascular processes
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