Abstract
Resolution capability of the linear structured illumination microscopy (SIM) plays a key role in its applications in physics, medicine, biology, and life science. Many advanced methodologies have been developed to extend the resolution of structured illumination by using subdiffraction‐limited optical excitation patterns. However, obtaining SIM images with a resolution beyond 40 nm at visible frequency remains as an insurmountable obstacle due to the intrinsic limitation of spatial frequency bandwidth of the involved materials and the complexity of the illumination system. Here, a low‐loss natural organic hyperbolic material (OHM) that can support record high spatial‐frequency modes beyond 50k 0, i.e., effective refractive index larger than 50, at visible frequencies is reported. OHM‐based speckle structured illumination microscopy demonstrates imaging resolution at 30 nm scales with enhanced fluorophore photostability, biocompatibility, easy to use and low cost. This study will open up a new route in super‐resolution microscopy by utilizing OHM films for various applications including bioimaging and sensing.
Highlights
Yeon Ui Lee, Clara Posner, Zhaoyu Nie, Junxiang Zhao, Shilong Li, Steven Edward Bopp, Gde Bimananda Mahardika Wisna, Jeongho Ha, Chengyu Song, Jin Zhang, Sui Yang, Xiang Zhang, and Zhaowei Liu*
We demonstrated an OHM-based super-resolution imaging technique based on the speckle structured illumination microscopy
The achieved precision of lateral resolution reaches down to 30 nm, which is more than 17 times spatial resolution improvement beyond the diffraction limit
Summary
On the other hand, predetermined speckles in OHM assisted illumination microscopy can represent much more prior information enabling improved image reconstruction with less measurements. This eventually can lead to higher imaging speed. An SEM image of Cos-7 cells cultured on a glass coverslip (Figure S6a, Supporting Information) shows overly bright and unstable intensity due to accumulated charges on the top surface of the glass. SEM images of Cos-7 cells cultured on the electrically conductive and biocompatible OHM substrate Information) show outstanding resolution and contrast over the entire field of view
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