Organic chromium modifies the expression of orexin and glucose transporters of ovarian in heat-stressed laying hens.

O Ozdemir,Z Tuzcu,K Sahin,C Orhan,M Tuzcu,N Sahin

doi:10.14715/cmb/2017.63.10.15

O Ozdemir, Z Tuzcu + Show 4 more

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https://doi.org/10.14715/cmb/2017.63.10.15

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Abstract

In this study, the effect of the supplemental organic chromium (Cr) forms on the expression of ovarian orexin(hypocretin), glucose transporters (GLUTs), heat shock proteins (HSPs) andnuclear factor-kappaB (NF-κB)were investigated in laying hens (HS). Laying hens (n=1800; 16-wk-old; Lohmann LSL-Lite) were allocated to 6 random groups according to a 2 × 3 factorial trial scheme with two different environmental temperatures [Thermoneutral (TN groups; at either 22±2 °C 24 h/d) and heat stress (HS groups; at 34±2 °C for 8 h/d, 08:00 to 17:00 h, followed by 22°C for 16 h for a period of 12 wks)], andhens reared under both environmental conditions were fed either a basal diet or the basal diet supplemented with 1.600 mg of chromium-picolinate (CrPic, 12.43% Cr) and 0.788 mg of chromium-histidinate (CrHis, 25.22% Cr) per kg of diet, delivering 200 µg elemental Cr per kg diet. HS groups showed decreased levels of orexin and GLUTs(GLUT1, GLUT4), and increased NFκB, HSP60, HSP70 and HSP90 levels compared to the TN groups in ovarian tissue of hens (P < 0.0001 for all).However, dietary chromium supplementation (CrPic-CrHis) increasedorexin and GLUTs levels and significantly reduced the NF-κB and HSPs levels making them closer to those of thermoneutral group (P < 0.0001).In conclusion, CrPic and CrHis showed supported the relief and treatment of stress complications.

Highlights

Heat stress is one of the most significant obstacles in the livestock industry, especially in poultry farms
In this study, the effect of the supplemental organic chromium (Cr) forms on the expression of ovarian orexin(hypocretin), glucose transporters (GLUTs), heat shock proteins (HSPs) andnuclear factor-kappaB (NF-κB)were investigated in laying hens (HS)
Evaluating the stress groups with each other, orexine (30.74%), GLUT1 (39.05%) and GLUT4 (49.62) levels significantly increased in the CrPic group compared to the control group (P

Summary

Introduction

Heat stress is one of the most significant obstacles in the livestock industry, especially in poultry farms. Heat stress compromises health and well-being but it negatively affects survival, performance and product quality [1,2]. It has been reported that heat stress in poultry affects yield properties thatinclude growth rate, feed conversion ratio, and body weight, and it, has negative effects on animal performance and product quality [3,4]. Orexins have been shown to regulate energy homeostasis (energy intake and consumption) [11], and both glucose and lipid metabolism [12].One of the most important features of orexin is that it is a stress modulator. The effect of the orexin system on the stress mechanism has not been clearly explained in many species [8]

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