Organic carbon and its fractions in paddy soil as affected by different nutrient and water regimes

Abstract

As an essential indicator of soil quality, soil organic carbon (SOC) and its fractions play an important role in many soil chemical, physical, and biological properties. A 4-year field experiment was conducted to determine the effects of different nutrient and water regimes on paddy soil organic carbon quality by measuring the major SOC fractions. Four nutrient regimes were compared: (i) control; (ii) chemical fertilizers only (CF), (iii) combined application of chemical fertilizers with farmyard manure (FYM) (CM), and (iv) combined application of chemical fertilizers and wheat straw (CS). Two soil water regimes included continuous waterlogging (CWL) and alternate wetting and drying (AWD). The total organic carbon (TOC) and total nitrogen (TN) in paddy soil were 40–60% and 37–67% higher in the combined organic sources and chemical fertilizers treatment against the sole chemical fertilizers treatment (CF), especially under continuous waterlogging (CWL). By fractionalizing SOC, it was observed that, under the water regimes of CWL, easily oxidizable carbon (EOC), particulate organic carbon (POC), light fraction organic carbon (LFOC), microbial biomass carbon (MBC), and mineralizable organic carbon (MNC) in the organically treated paddy soil were significantly ( P<0.05) lower, as compared with alternate wetting and drying (AWD). Especially for CM treatment, EOC, POC, LFOC, MBC, and MNC in the paddy soils under the regime of CWL were 23.5%, 32.7%, 16.3%, 56.8% and 25.1% lower than those by AWD, respectively. The proportions of EOC, POC, LFOC, MBC and MNC as a percent of TOC in the CWL were lower than those in the AWD, especially for the CM treatment. In the water regime of CWL, no significant differences were seen in the corresponding proportion of all the investigated organic fractions to soil total organic carbon (TOC) among the three fertilization treatments, whereas in the AWD, the corresponding proportions of different carbon fractions to TOC in the organic fertilizer treatments were significantly ( P<0.05) higher than those in the chemical fertilizer treatment. Under continuous waterlogging, the proportion of soil water stable aggregate >250 μm (WSA) decreased by 42–45% and clay dispersion ratio ( R CD) increased by 12–38%, as compared to the water regimes of AWD, when FYM or wheat straw was incorporated into paddy soil. Correlation analysis showed that, under the water regimes of AWD, WSA was significantly and positively related to EOC, LFOC and POC with the coefficients ( r) of 0.822, 0.889, 0.912 ( P<0.01), respectively. R CD was negatively correlated to EOC, LFOC and POC with the r=−0.796, −0.854, and −0.897 ( P<0.01), respectively, under AWD. Under the water regimes of CWL, there were no significant ( P<0.05) correlations between WSA as well as R CD and any organic carbon fraction except POC.