Abstract
Organelle genome diversity was analysed in interspecific potato somatic hybrids using chloroplast (cp)- and mitochondrial (mt)-specific molecular markers. Out of total 25 markers (15 cpDNA and 10 mtDNA) tested in total 16 samples, only four mtDNA primers (rpS14/cob, Nsm2, ALM4/ALM5 and ALM6/ALM7) detected polymorphism, whereas other primers were monomorphic. Cluster analysis showed higher genetic diversity among the genotypes by mtDNA profiles than that by cpDNA. Ten haplotypes were grouped by cluster analysis comprised of maximum seven genotypes in haplotype no. 3. Monomorphic markers did not reveal variability in our samples and suggest highly conserved organelle genomic regions. New genomic arrangements were observed in the somatic hybrids for mt polymorphic loci. Our study suggests that somatic hybrids are comprised of diverse cytoplasm types consisting predominantly of T-, W-, and C-, with a few A- and S-type chloroplast, and α-, β- and γ-type mitochondrial genome, and have unique potential to widen the cultivated potato gene pool by breeding methods.
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