Abstract

Under electrophoretic conditions, arrays of fine, cytoplasmic organelles with a fixed positive charge concentrate in a consistently oriented pattern around the cathode region of the nuclear envelope. Their concentration profile agrees with a theoretical model for the electrophoretic migration of particles onto the surface of a spherical membrane. Inside the cell nucleus an electrokinetic mobility occurs in negatively charge nucleoli, with exponential movement toward the anode region. From a velocity-size relationship the nature of the nucleolus mobility is consistent with the electrokinetic theory of uniformly charged particles immersed in a dielectric medium, and responding to an induced dipole field on the nucleus. The introduction of fluorescein provided a delineation of polarity related movement of chloroplasts during intracellular transductions.

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