Abstract

The behaviour of cell nuclei and organelle nucleoids (organelle nuclei) was studied in the root apical meristem of 3-d-old seedlings of Arabidopsis thaliana (Col.). Samples were embedded in Technovit 7100 resin, cut into thin sections and stained with 4'-6-diamidino-2-phenylindole (DAPI) for observation of DNA. DNA synthesis in cell nuclei and organelle nucleoids was investigated using the incorporation of [ 3 H] thymidine or 5-bromo-2'-deoxyuridine (BrdU). Incorporated [ 3 H] thymidine and BrdU were detected by microautoradiography or immunofluorescence microscopy, respectively. Central cells and cells just above the central cells of the quiescent centre (QC) showed an extremely low activity of DNA synthesis

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