Abstract

A cDNA library established from polyA+RNA from tubers of potato (Solanum tuberosum) was analysed for sequences specifically transcribed in tubers. Screening more than 8000 cDNA clones in a differential hybridization assay using RNA from leaves, roots, stems and tubers resulted in four different classes of cDNA clones. These could be categorized further into two groups according to the size distribution of mRNAs to which they hybridize. Thus the first group comprising cDNA clones pcT700, pcT800 and pcT1500 were in each case complementary to a single RNA species. Hybrid select/in vitro translation data indicate that one of these clones, i.e. pcT1500, is derived from an RNA encoding the major storage protein of potato tubes, i.e. a 40 kd species (called “patatin”).

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