Abstract
The liver, the largest organ of the human body, is a multifunctional organ with various metabolic activities that plays a fundamental role in maintaining the body and in sustaining life. Although the liver has great regenerative capacity and recovery, the damage caused by chronic diseases such as cancer or viral infections can lead to permanent loss of liver function. Studies on the mechanism of liver disease, have focused on the selection of cell and tissue culture techniques, including strategies based on in vitro models. The organ culture is a promising tool for the study of liver diseases, because it can mimic the complex of the microenvironment in vivo using a three-dimensional model of human liver tissue. These models allow a better study of the specific functions of the liver. In this context, we have analyzed the development of a hepatocarcinoma, obtained by inoculating a murine hepatocarcinoma cell line, Hepa 1/A1s, in the liver of 10 mice of the strain C57BL / 6. After 20 days from the inoculation, the portion of liver invaded by the tumor was removed from the animals and cultured. A group of 5 liver explants were used as a control and other 5 explants were cultured for 4 weeks in a complete medium containing 10% Citozym , a food supplement with reported antioxidant properties. The cancer-invaded hepatic lobes, treated with Citozym , showed a clear reduction of the weight and the volume of the hepatic tumors, when compared with the control explants.
Highlights
Cirrhosis and hepatocellular carcinoma (HCC) have been put to the fifth most common cause of death
Liver cirrhosis is the bridge between the chronic inflammation of the liver to HCC, any kind of chronic inflammatory disease that affects this organ, such as viral hepatitis or alcoholic hepatitis can lead to this form of cancer [1]
We have shown that cultured liver explants, invaded by HCC, obtained from C57BL / 6 mice inoculated intraperitoneally with cells of the line Hepa 1/A1s, it is sufficiently viable for long times, in order to assess the antineoplastic action of a mixture of antioxidants, present in a nutritional supplement (CIZ)
Summary
Cirrhosis and hepatocellular carcinoma (HCC) have been put to the fifth most common cause of death. Liver cirrhosis is the bridge between the chronic inflammation of the liver to HCC, any kind of chronic inflammatory disease that affects this organ, such as viral hepatitis or alcoholic hepatitis can lead to this form of cancer [1]. The animal models used for the trials lead to interesting results, but refuted by many, both for the use of animals and for the results considered not comparable with those obtainable on the human [2]. New initiatives seek to address this problem by funding the development of physiologically relevant models of human organs [4].
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