Orderly assembly underpinning built-in asymmetry in the yeast centrosome duplication cycle requires cyclin-dependent kinase.

Marco Geymonat,Qiuran Peng,Marisa Segal,Sue L Jaspersen,Zulin Yu,Zhiang Guo,Jay R Unruh

doi:10.7554/elife.59222

Abstract

Asymmetric astral microtubule organization drives the polarized orientation of the S. cerevisiae mitotic spindle and primes the invariant inheritance of the old spindle pole body (SPB, the yeast centrosome) by the bud. This model has anticipated analogous centrosome asymmetries featured in self-renewing stem cell divisions. We previously implicated Spc72, the cytoplasmic receptor for the gamma-tubulin nucleation complex, as the most upstream determinant linking SPB age, functional asymmetry and fate. Here we used structured illumination microscopy and biochemical analysis to explore the asymmetric landscape of nucleation sites inherently built into the spindle pathway and under the control of cyclin-dependent kinase (CDK). We show that CDK enforces Spc72 asymmetric docking by phosphorylating Nud1/centriolin. Furthermore, CDK-imposed order in the construction of the new SPB promotes the correct balance of nucleation sites between the nuclear and cytoplasmic faces of the SPB. Together these contributions by CDK inherently link correct SPB morphogenesis, age and fate.

Highlights

You should state the statistical method of sample size computation and any required assumptions
If no explicit power analysis was used, you should describe how you decided what sample size to use Please outline where this information can be found within the submission, or explain why this information doesn’t apply to your submission: The information has been incorporated in each figure and/or figure legend and in Materials and Methods
High-throughput sequence data should be uploaded before submission, with a private link for reviewers provided

Summary

Introduction

You should state the statistical method of sample size computation and any required assumptions If no explicit power analysis was used, you should describe how you decided what sample (replicate) size (number) to use Please outline where this information can be found within the submission (e.g., sections or figure legends), or explain why this information doesn’t apply to your submission: The information has been incorporated in each figure and/or figure legend and in Materials and Methods.

Results

Conclusion