Abstract
The possibility of using anthers for initiating in vitro cultures of Rhynchostylis retusa (Orchidaceae), has been positively tested. The study was designed to study the effects of physiological status, stage of microspore development and pre-cold treatment on androgenic response in R.retusa. The anthers from open flowers (2 days of anthesis) in tetrad stage failed to respond despite variations in the chemical regime; whereas those from unopened buds (1.25-1.35cm long) with an intact operculum & pre-cold treatment with microspores in early & late-uninucleate decussate regenerated provided their nutritional complexities were satisfied through an exogeneous supply of Peptone (2mg/l) in the BAP (10mg/l) and NAA enriched Sharma medium. The best response was obtained when a 24hrs cold treatment was employed at 4°C in darkness. The complete plantlet (2-3 leaves &1-2 roots) was formed in 24wks.The plantlet was accilimatized & the survival rate is 70%.
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