Abstract

Orange (pulp) and pineapple wastes were used as substrates for citric acid production by two strains of Aspergillus niger. A. niger strains NRRL 567 and 328 produced the maximum amount of citric acid (57.6% and 55.4%, respectively) at a moisture content of 38.9% in orange waste and the highest yields of 46.4% and 45.4% citric acid in pineapple waste at moisture contents of 54.4% and 63.4 %, respectively. The addition of 1–3% methanol to the substrates resulted in reduction in yield in both cases.

Highlights

  • E Citric acid producing strains of A. niger, NRRL 567 and 328, were obtained from Dr C.W

  • The molasses, and apple pomace for citric acid pro- A. niger strains NRRL 567 and 328, previously yields of citric acid produced were 46.4% and duction has been reviewed by Kapoor et al.[1] grown on potato dextrose agar slant at 28°C for 45.4% at moisture contents of 54.8% and

  • Apple pomace has been used as an energy 7 days, was added to pineapple and orange 63.4%, using strains NRRL 328 and 567, source in alleviating the waste disposal prob- wastes each in a conical flask

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Summary

Introduction

E Citric acid producing strains of A. niger, NRRL 567 and 328, were obtained from Dr C.W. World’s output is produced using Aspergillus The flasks were cooled and inoculated with 1 The orange and pineapple wastes had initial niger, A. wentii, A. clavatus, Penicillium mL suspension of A. niger strains NRRL 567 moisture contents of 82.3% and 79.6%, and pH The use of sucrose, methanol on the yield of citric acid niger strains NRRL 328 and 567 at moisture starch from various sources, cane and beet One milliliter of each of the suspensions of levels of 22.5-38.9%.

Results
Conclusion
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