Abstract
The objective of this study was to evaluate the effects of oral fish cartilage hydrolysate (FCH) on symptoms and joint tissue structure in rat developing osteoarthritis induced surgically. Osteoarthritis was induced in the right knee of mature male Lewis rats (n = 12/group) by surgical transection of the anterior cruciate ligament (ACLT) combined with partial medial meniscectomy (pMMx). Two weeks after surgery, rats were treated orally with either control (sterile H2O) or FCH for four weeks. Pain and function were assessed by dynamic weight‐bearing test (incapacitance test), electronic Von Frey (EVF; hindpaw allodynia threshold), and pressure algometer (knee allodynia threshold). Time and groups differences at each time point were evaluated using a mixed model. The histological features were evaluated eight weeks after surgery using OARSI score. Mann–Whitney test nonparametric test was applied to compare OARSI score. ACTL/pMMx surgery significantly reduced weight‐bearing and increased allodynia and sensitivity thresholds of the operated paw/knee. Globally, FCH improved these parameters faster, but no significant difference between control and FCH groups was observed. Eight weeks after surgery, rats developed moderate OA lesions. Compared with control, FCH did not significantly modify OA lesion severity assessed using the OARSI score. In this mechanically induced OA model, 4 weeks of supplementation with FCH had no significant effect on cartilage lesion, but tends to accelerate pain relief and joint function recovery. This positive trend may have opened the way for further investigation of FCH as potential treatment of joint discomfort associated with OA.
Highlights
Osteoarthritis (OA) is a common painful condition that imposes a substantial burden on individuals, healthcare systems and society
Several food supplements have been studied in variable quality randomized clinical trials, and the overall analysis including all trials showed that some supplements provide moderate and clinically meaningful short-term effects on pain and function in patients with hand, hip, or knee OA (Lim et al, 2019; Liu et al, 2018)
ACTL/pMMx surgery induced a significant decrease in the pain threshold in the operated knee at W1 in both study groups: −143.4 g, p < .001; fish cartilage hydrolysate (FCH): -215.3 g, p < .001) and progressively returned to normal level with time (Figure 4a)
Summary
Osteoarthritis (OA) is a common painful condition that imposes a substantial burden on individuals, healthcare systems and society. Its prevalence increases due to population aging and growing number of overweight people (Johnson & Hunter, 2014; Loeser et al, 2012) At this time, symptoms-focused OA management requires an association of nonpharmacological and pharmacological modalities (i.e., analgesic drugs or intra-articular injections of corticosteroid or hyaluronic acid (HA)) should be to relieve pain and to improve joint function and quality of life of patient with limb OA (2014; Hochberg et al, 2012; Jevsevar, 2013; McAlindon et al, 2014; Michael et al, 2010). Before their clinical use, their effects have to be investigated in in vitro and in vivo preclinical models In this preclinical study, we have tested a FCH on the knee pain and mobility of rat with OA induced experimentally by the section of the anterior cruciate ligament (ACLT) associated with a partial medial meniscectomy (pMMx). It was the first time that this FCH was tested in animal developing mechanically induced OA
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