Abstract
[Objective] To investigate the effect of angiogenesis and collagen synthesis in peri-infarct area of the L-Arginine therapy for acute myocardial infarction rats. [Methods] The acute myocardial infarction rats model was established by ligation of the left anterior descending of coronary artery. Thirty male Sprague-Dawley rats were randomly divided into three groups: L-Arginine group, sham group, normal saline group (NS group). Four weeks after ligation, cardiac function, scar area, plasma concentration of BNP, angiogenesis and arteriogenesis, myocardial collagen I and eNOS protein, the mRNA expression of collagen I were studied. Echocardiography, Masson staining, enzyme-linked immunosorbent assay (ELISA), immunehistochemistry, western blot and quantitative polymerase chain (qPCR) reaction were performed. [Results] Four weeks after ligation, compared with the control group, LVEF, LVFS were higher in L-Arginine group, While LVEDD and LVESD decreased (P < 0.01). Average scar percentage and plasma concentration of BNP were lower in L-Arginine group (P < 0.01). The CD31-positive microvessels and α-SMA positive microvessels in peri-infarct area were higher in L-Arginine group (P < 0.01), while collagen I protein and mRNA expression was decreased in this group (P < 0.01). [Conclusions] L-Arginine improves cardiac function and reduces infarction size in AMI rats, the possible mechanism is related to dual function of promoting angiogenesis and arteriogenesis, regulating collagen I expression is also one of the important mechanisms.
Highlights
To reduce the mortality of patients with acute myocardial infarction (AMI) and improve their cardiac function and clinical outcomes, the use of adjuvant therapy to improve the prognosis of AMI, including stem cell transplantation and cytokine therapy, has gradually been studied by scholars [1]
There were no differences among the three groups in the values of left ventricular end-diastolic dimension (LVEDD), left ventricular end-systolic dimension (LVESD), and left ventricular ejection fraction (LVEF), (P > 0.05)
The results showed that L-Arginine intervention could improve left ventricular ejection fraction, reduce infarct size, increase capillary and arteriolar regeneration in rats with acute myocardial infarction
Summary
To reduce the mortality of patients with acute myocardial infarction (AMI) and improve their cardiac function and clinical outcomes, the use of adjuvant therapy to improve the prognosis of AMI, including stem cell transplantation and cytokine therapy, has gradually been studied by scholars [1]. Therapeutic angiogenesis is an attractive alternative approach to the treatment of myocardial ischemia, in which the induction of angiogenesis is used to preserve cardiac function by promoting the growth of new blood vessels and facilitating the production of new cardiomyocytes of the ischemic region [2, 3]. Among these substances that may promote blood vessel growth, L-Arginine has become a hot topic for its dual role in promoting angiogenesis and improving vascular endothelial function [4]. Whether L-Arginine can improve the ventricular remodeling after
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